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Article

Extended RAS and BRAF Mutation Analysis Using Next-Generation Sequencing

8 May 2015

Abstract excerpt

Somatic mutations in KRAS, NRAS, and BRAF genes are related to resistance to anti-EGFR antibodies in colorectal cancer. We have established an extended RAS and BRAF mutation assay using a next-generation sequencer to analyze these mutations. Multiplexed deep sequencing was performed to detect somatic mutations within KRAS, NRAS, and BRAF, including minor mutated components. We first validated the technical...

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PMID: 25954997
DOI: 10.1371/journal.pone.0121891

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