Article
Development and Analytical Validation of a DNA Dual-Strand Approach for the US Food and Drug Administration-Approved Next-Generation Sequencing-Based Praxis Extended RAS Panel for Metastatic Colorectal Cancer Samples.
The Journal of molecular diagnostics : JMD - 1 Feb 2020
Udar Nitin, Iyer Anita, Porter Margaret, Haigis Robert, Smith Shannon, Dhillon Shivani, Meier Kristen, Ward Diane, Lu Jing, Wenz Paul, Buchner Leonard, Dunn Tamsen, Wise Aaron, Mueller Amy, Gutekunst Karen
Abstract excerpt
A next-generation sequencing method was developed that can distinguish single-stranded modifications from low-frequency somatic mutations present on both strands of DNA in formalin-fixed paraffin-embedded colorectal cancer samples. We applied this method for analytical validation of the Praxis Extended RAS Panel, a US Food and Drug Administration-approved companion diagnostic for panitumumab, on the Illumina...
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