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Article

Mutation discovery by targeted genomic enrichment of multiplexed barcoded samples.

Nature methods - 1 Nov 2010

Nijman Isaäc J, Mokry Michal, van Boxtel Ruben, Toonen Pim, de Bruijn Ewart, Cuppen Edwin

Abstract excerpt

Targeted genomic enrichment followed by next-generation DNA sequencing has dramatically increased efficiency of mutation-discovery efforts. We describe a protocol for genomic enrichment of pooled barcoded samples in a single assay that increases experimental flexibility and efficiency. We screened 770 genes (1.4 megabases) in thirty N-ethyl-N-nitrosourea (ENU)-mutagenized rats and identified known variants at...

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PMID: 20953175
DOI: 10.1038/nmeth.1516

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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