Article
Melting curve analysis after T allele enrichment (MelcaTle) as a highly sensitive and reliable method for detecting the JAK2V617F mutation.
PloS one - 1 Jan 2015
Morishita Soji, Takahashi Kochi, Araki Marito, Hironaka Yumi, Sunami Yoshitaka, Edahiro Yoko, Tsutsui Miyuki, Ohsaka Akimichi, Tsuneda Satoshi, Komatsu Norio
Abstract excerpt
Detection of the JAK2V617F mutation is essential for diagnosing patients with classical myeloproliferative neoplasms (MPNs). However, detection of the low-frequency JAK2V617F mutation is a challenging task due to the necessity of discriminating between true-positive and false-positive results. Here, we have developed a highly sensitive and accurate assay for the detection of JAK2V617F and named it melting curve...
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