Article
Optimization of a Low-Cost, Sensitive PNA Clamping PCR Method for JAK2 V617F Variant Detection.
The journal of applied laboratory medicine - 1 Jul 2020
Di Francia Raffaele, Crisci Stefania, Muto Tommaso, Giancola Concetta, Petriccone Luigi, Catapano Oriana, Cummarro Annunziata, Pinto Antonio, Frigeri Ferdinando
Abstract excerpt
BACKGROUND: The JAK2 V617F variant is diagnostic for myeloproliferative neoplasms, a group of clonal disorders of hematopoietic stem and progenitor cells. Although several approaches have been developed to detect the variant, a gold standard diagnostic method has not yet been defined. We describe a simple, fast, and cost-effective PCR-based approach that enhances test specificity and sensitivity by blocking the...
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