Article

Identification of theJAK2V617F Mutation in Chronic Myeloproliferative Disorders Using FRET Probes and Melting Curve Analysis

2006-04-01

Abstract excerpt

We developed and validated a real-time polymerase chain reaction assay using fluorescent hybridization probes and melting curve analysis to identify the JAK2V617F mutation, which is implicated in a substantial proportion of chronic myeloproliferative disorders (CMPDs). DNA from 161 samples was isolated from peripheral blood granulocytes and formalin-fixed bone marrow clot sections in patients with CMPDs and withou...

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Literature Corpus work: b7374c48-db60-5e36-ab5a-c2b5105a1a56
DOI: 10.1309/tk0xl917xk2vlrpq

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