Article
A novel, highly sensitive and rapid allele-specific loop-mediated amplification assay for the detection of the JAK2V617F mutation in chronic myeloproliferative neoplasms.
Haematologica - 1 Sept 2012
Minnucci Giulia, Amicarelli Giulia, Salmoiraghi Silvia, Spinelli Orietta, Guinea Montalvo Marie Lorena, Giussani Ursula, Adlerstein Daniel, Rambaldi Alessandro
Abstract excerpt
BACKGROUND: The identification of the JAK2V617F mutation is mandatory in the diagnostic work-up of Philadelphia chromosome-negative myeloproliferative neoplasms. Several molecular techniques to detect this mutation are currently available, but each of them has some limits. DESIGN AND METHODS: We set up a novel molecular method for the identification of the JAK2V617F mutation based on an allele-specific...
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