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Article

Directed evolution of λ integrase activity and specificity by genetic derepression.

Protein engineering, design & selection : PEDS - 1 Jul 2015

Siau Jia Wei, Chee Sharon, Makhija Harshyaa, Wai Cho Mar Myint, Chandra Shree Harsha Vijaya, Peter Sabrina, Dröge Peter, Ghadessy Farid J

Abstract excerpt

Advances in genome engineering are attendant on the development of novel enzyme variants with programed substrate specificities and improved activity. We have devised a novel selection method, wherein the activity of a recombinase deletes the gene encoding an inhibitor of an enzyme conferring a s...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 25787692
DOI: 10.1093/protein/gzv015

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-25787692-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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