Article

An Orthogonal T7 Replisome for Continuous Hypermutation and Accelerated Evolution in <i>E. coli</i>

2024-07-26

Abstract excerpt

Systems that perform continuous hypermutation of designated genes without compromising the integrity of the host genome can dramatically accelerate the evolution of new or enhanced protein functions. We describe an orthogonal DNA replication system in E. coli based on the controlled expression of the replisome of bacteriophage T7. The system replicates circular plasmids that enable high transformation efficiencie...

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Identifiers and source

Literature Corpus work: 5598506a-f117-5124-a228-a317ce3daec4
DOI: 10.1101/2024.07.25.605042

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