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Directed evolution of recombinase specificity by split gene reassembly.

Nucleic acids research - 1 Jul 2010

Gersbach Charles A, Gaj Thomas, Gordley Russell M, Barbas Carlos F

Abstract excerpt

The engineering of new enzymes that efficiently and specifically modify DNA sequences is necessary for the development of enhanced gene therapies and genetic studies. To address this need, we developed a robust strategy for evolving site-specific recombinases with novel substrate specificities. In this system, recombinase variants are selected for activity on new substrates based on enzyme-mediated reassembly of...

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Identifiers

PMID: 20194120
DOI: 10.1093/nar/gkq125
PMCID: PMC2896519

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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