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Article

Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I.

Proceedings of the National Academy of Sciences of the United States of America - 19 Aug 2003

Camps Manel, Naukkarinen Jussi, Johnson Ben P, Loeb Lawrence A

Abstract excerpt

We present a system for random mutagenesis in Escherichia coli for the evolution of targeted genes. To increase error rates of DNA polymerase I (Pol I) replication, we introduced point mutations in three structural domains that govern Pol I fidelity. Expression of error-prone Pol I in vivo results in strong mutagenesis of a target sequence encoded in a Pol I-dependent plasmid (8.1 x 10-4 mutations per bp, an...

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Identifiers

PMID: 12909725
DOI: 10.1073/pnas.1333928100
PMCID: PMC187833

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Source receipt: pubmed, retrieved 7 Sept 2026.

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