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Article

An orthogonal T7 replisome for continuous hypermutation and accelerated evolution in E. coli.

Science (New York, N.Y.) - 7 Aug 2025

Diercks Christian S, Sondermann Philipp, Rong Cynthia, Gillis Thomas G, Ban Yahui, Wang Celine, Dik David A, Schultz Peter G

Abstract excerpt

Systems that perform continuous hypermutation of designated genes without compromising the integrity of the host genome can substantially accelerate the evolution of new or enhanced protein functions. We describe an orthogonal DNA replication system in Escherichia coli based on the controlled expression of the replisome of bacteriophage T7 (T7-ORACLE). The system replicates circular plasmids that enable high...

Read the complete abstract on PubMed

Topics

Bacteriophage T7
beta-Lactamases
Cephalosporins
Directed Molecular Evolution
DNA Replication
DNA-Directed DNA Polymerase

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Escherichia coli

Multienzyme Complexes

Mutation

Mutation Rate

Plasmids

Protein Engineering

Viral Proteins

DNA-Directed RNA Polymerases

Mutagenesis

Replisomes

Source

Identifiers

PMID: 40773556
DOI: 10.1126/science.adp9583

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