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Article

The structure of SpnF, a standalone enzyme that catalyzes [4 + 2] cycloaddition.

Nature chemical biology - 1 Apr 2015

Fage Christopher D, Isiorho Eta A, Liu Yungnan, Wagner Drew T, Liu Hung-wen, Keatinge-Clay Adrian T

Abstract excerpt

In the biosynthetic pathway of the spinosyn insecticides, the tailoring enzyme SpnF performs a [4 + 2] cycloaddition on a 22-membered macrolactone to forge an embedded cyclohexene ring. To learn more about this reaction, which could potentially proceed through a Diels-Alder mechanism, we determined the 1.50-Å-resolution crystal structure of SpnF bound to S-adenosylhomocysteine. This sets the stage for advanced...

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Identifiers

PMID: 25730549
DOI: 10.1038/nchembio.1768
PMCID: PMC4366278

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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