Article
Effect of the enzyme and PCR conditions on the quality of high-throughput DNA sequencing results.
Scientific reports - 27 Jan 2015
Brandariz-Fontes Claudia, Camacho-Sanchez Miguel, Vilà Carles, Vega-Pla José Luis, Rico Ciro, Leonard Jennifer A
Abstract excerpt
Library preparation protocols for high-throughput DNA sequencing (HTS) include amplification steps in which errors can build up. In order to have confidence in the sequencing data, it is important to understand the effects of different Taq polymerases and PCR amplification protocols on the DNA molecules sequenced. We compared thirteen enzymes in three different marker systems: simple, single copy nuclear gene and...
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