Optimization of the genotyping-by-sequencing strategy for population genomic analysis in conifers.

Abstract excerpt

Flexibility and low cost make genotyping-by-sequencing (GBS) an ideal tool for population genomic studies of nonmodel species. However, to utilize the potential of the method fully, many parameters affecting library quality and single nucleotide polymorphism (SNP) discovery require optimization, especially for conifer genomes with a high repetitive DNA content. In this study, we explored strategies for effective...

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• DNA, Plant
• DNA Restriction Enzymes
• Genotyping Techniques
• Metagenomics
• Polymorphism, Single Nucleotide