Article
Genotyping mitochondrial DNA single nucleotide polymorphisms by PCR ligase detection reactions.
Clinical chemistry and laboratory medicine - 1 Apr 2010
Luo Yongjun, Tang Sha, Gao Wenxiang, Chen Li, Yang Xiaohong, Huang Taosheng, Gao Yuqi
Abstract excerpt
BACKGROUND: The identification of human mitochondrial DNA (mtDNA) sequence variations, especially single nucleotide polymorphisms (SNPs), is important for many applications. The PCR-ligase detection reaction (LDR) method can reduce false-positives and eliminate the need for both post-PCR and post-ligation purifications in SNP analyses. In addition, it has been successfully employed to detect point mutations in...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.