Article
A method for counting PCR template molecules with application to next-generation sequencing.
Nucleic acids research - 1 Jul 2011
Casbon James A, Osborne Robert J, Brenner Sydney, Lichtenstein Conrad P
Abstract excerpt
Amplification by polymerase chain reaction is often used in the preparation of template DNA molecules for next-generation sequencing. Amplification increases the number of available molecules for sequencing but changes the representation of the template molecules in the amplified product and introduces random errors. Such changes in representation hinder applications requiring accurate quantification of template...
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