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Article

A prolific and robust whole-genome genotyping method using PCR amplification via primer-template mismatched annealing.

Journal of integrative plant biology - 1 Mar 2023

Zhao Sheng, Zhang Cuicui, Wang Liqun, Luo Minxuan, Zhang Peng, Wang Yue, Malik Waqar Afzal, Wang Yue, Chen Peng, Qiu Xianjin, Wang Chongrong, Lu Hong, Xiang Yong, Liu Yuwen, Ruan Jue, Qian Qian, Zhi Haijian, Chang Yuxiao

Abstract excerpt

Whole-genome genotyping methods are important for breeding. However, it has been challenging to develop a robust method for simultaneous foreground and background genotyping that can easily be adapted to different genes and species. In our study, we accidently discovered that in adapter ligation-mediated PCR, the amplification by primer-template mismatched annealing (PTMA) along the genome could generate...

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PMID: 36269601
DOI: 10.1111/jipb.13395

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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