Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Modifications of Kompetitive Allele-Specific PCR (KASP) Genotyping for Detection of Rare Alleles.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023

Brusa Anthony, Patterson Eric, Fleming Margaret

Abstract excerpt

KASP is commonly used to genotype bi-allelic SNPs and In/Dels, and the standard protocol works well when both alleles are nearly equally prevalent in the DNA template. To detect rare alleles in bulked samples or to distinguish more than three genotypes, such as tri-allelic loci or mutations across orthologous genes in polyploids, adjustments to the protocol and/or data analysis are required. In this chapter, we...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Alleles
Share to Genotype
Share to Nucleic Acid Amplification Techniques

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Polymerase Chain Reaction

Share to Polymorphism, Single Nucleotide

Share to Polyploidy

Source

Identifiers

PMID: 36781642
DOI: 10.1007/978-1-0716-3024-2_12

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Modifications of Kompetitive Allele-Specific PCR (KASP) Genotyping for Detection of Rare Alleles.PMID 36781642Read ↗

Development of Breeder-Friendly KASP Markers from Genome-Wide Association Studies Results.Semantic proximity Read ↗

KASP: a high-throughput genotyping system and its applications in major crop plants for biotic and abiotic stress tolerance.Semantic proximity Read ↗

SNP genotyping: the KASP assay.Semantic proximity Read ↗

Penta-Primer Amplification Refractory Mutation System (PARMS) with Direct PCR-Based SNP Marker-Assisted Selection (D-MAS).Semantic proximity Read ↗

A technical platform for PCR-based SNP screening in cereals and other crops.Semantic proximity Read ↗

Overcoming polyploidy pitfalls: a user guide for effective SNP conversion into KASP markers in wheat.Semantic proximity Read ↗

Identification of diagnostic KASP-SNP markers for routine breeding activities in yam (Dioscorea spp.).Semantic proximity Read ↗

Advantages of Amplifluor-like SNP markers over KASP in plant genotyping.Semantic proximity Read ↗

Effect of the enzyme and PCR conditions on the quality of high-throughput DNA sequencing results.Semantic proximity Read ↗

Allele-Specific Mutation Genotyping with Mismatches in Primer Design.Semantic proximity Read ↗

PCR Allele Competitive Extension (PACE) Workflow for Genotyping by SNP Identification.Semantic proximity Read ↗

An innovative SNP genotyping method adapting to multiple platforms and throughputs.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.