Article
Expression and purification of the N-terminal regulatory domain of Protein Kinase C for biophysical studies.
Protein expression and purification - 1 Jun 2015
Cole Taylor R, Igumenova Tatyana I
Abstract excerpt
We report the protocol for heterologous expression and purification of the N-terminal regulatory region of two Protein Kinase C (PKC)(1) isozymes, one conventional and one novel. Previous studies of these domains relied almost exclusively on the fusion constructs with high-molecular-weight solubility fusion partners such as GST and MBP. We developed experimental procedures that enabled us to overcome challenges...
Topics
- Amino Acid Sequence
- Animals
- Cloning, Molecular
- Escherichia coli
- Gene Expression
- Mice
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Nuclear Magnetic Resonance, Biomolecular
- Osmolar Concentration
- Plasmids
- Protein Kinase C
- Protein Kinase C-alpha
- Protein Kinase C-delta
- Protein Structure, Tertiary
- Rats
- Recombinant Fusion Proteins