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Article

Expression and biophysical analysis of two double-transmembrane domain-containing fragments from a yeast G protein-coupled receptor.

Biopolymers - 1 Jan 2008

Cohen Leah S, Arshava Boris, Estephan Racha, Englander Jacqueline, Kim Heejung, Hauser Melinda, Zerbe Oliver, Ceruso Marco, Becker Jeffrey M, Naider Fred

Abstract excerpt

Fragments of G protein-coupled receptors (GPCRs) are widely used as models to investigate these polytopic integral-membrane, signal-transducing molecules, but have proven difficult to prepare in quantities necessary for NMR analyses. We report on the biosynthesis of two double transmembrane (TM) containing fragments of Ste2p, the alpha-factor GPCR from the yeast Saccharomyces cerevisiae. Ste2p(G31-T110) [TM1-TM2]...

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PMID: 18260136
DOI: 10.1002/bip.20950

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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