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Article

Universal platform for the generation of thermostabilized GPCRs that crystallize in LCP.

Nature protocols - 1 Mar 2022

Schöppe Jendrik, Ehrenmann Janosch, Waltenspühl Yann, Plückthun Andreas

Abstract excerpt

Structural studies of G-protein-coupled receptors (GPCRs) are often limited by difficulties in obtaining well-diffracting crystals suitable for high-resolution structure determination. During the past decade, crystallization in lipidic cubic phase (LCP) has become the most successful and widely used technique for obtaining such crystals. Despite often intense efforts, many GPCRs remain refractory to...

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PMID: 35140409
DOI: 10.1038/s41596-021-00660-9

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