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Article

Active destabilization of base pairs by a DNA glycosylase wedge initiates damage recognition.

Nucleic acids research - 1 Jan 2015

Kuznetsov Nikita A, Bergonzo Christina, Campbell Arthur J, Li Haoquan, Mechetin Grigory V, de los Santos Carlos, Grollman Arthur P, Fedorova Olga S, Zharkov Dmitry O, Simmerling Carlos

Abstract excerpt

Formamidopyrimidine-DNA glycosylase (Fpg) excises 8-oxoguanine (oxoG) from DNA but ignores normal guanine. We combined molecular dynamics simulation and stopped-flow kinetics with fluorescence detection to track the events in the recognition of oxoG by Fpg and its mutants with a key phenylalanine residue, which intercalates next to the damaged base, changed to either alanine (F110A) or fluorescent reporter...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA-(Apurinic or Apyrimidinic Site) Lyase

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Identifiers

PMID: 25520195
DOI: 10.1093/nar/gku1300
PMCID: PMC4288190

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