Article
Two glycosylase families diffusively scan DNA using a wedge residue to probe for and identify oxidatively damaged bases.
Proceedings of the National Academy of Sciences of the United States of America - 20 May 2014
Nelson Shane R, Dunn Andrew R, Kathe Scott D, Warshaw David M, Wallace Susan S
Abstract excerpt
DNA glycosylases are enzymes that perform the initial steps of base excision repair, the principal repair mechanism that identifies and removes endogenous damages that occur in an organism's DNA. We characterized the motion of single molecules of three bacterial glycosylases that recognize oxidized bases, Fpg, Nei, and Nth, as they scan for damages on tightropes of λ DNA. We find that all three enzymes use a key...
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