Article
Hinge residue I174 is critical for proper dNTP selection by DNA polymerase beta.
Biochemistry - 23 Mar 2010
Yamtich Jen, Starcevic Daniela, Lauper Julia, Smith Elenoe, Shi Idina, Rangarajan Sneha, Jaeger Joachim, Sweasy Joann B
Abstract excerpt
DNA polymerase beta (pol beta) is the key gap-filling polymerase in base excision repair, the DNA repair pathway responsible for repairing up to 20000 endogenous lesions per cell per day. Pol beta is also widely used as a model polymerase for structure and function studies, and several structural regions have been identified as being critical for the fidelity of the enzyme. One of these regions is the hydrophobic...
Topics
- Animals
- Base Pair Mismatch
- Base Sequence
- DNA
- DNA Polymerase beta
- Isoleucine
- Molecular Dynamics Simulation
- Mutagenesis
- Mutation
- Nucleotides
- Protein Conformation