Article
Multigene knockout utilizing off-target mutations of the CRISPR/Cas9 system in rice.
Plant & cell physiology - 1 Jan 2015
Endo Masaki, Mikami Masafumi, Toki Seiichi
Abstract excerpt
The clustered regularly interspaced short palindromic repeat (CRISPR)-associated endonuclease 9 (CRISPR/Cas9) system has been demonstrated to be a robust genome engineering tool in a variety of organisms including plants. However, it has been shown that the CRISPR/Cas9 system cleaves genomic DNA sequences containing mismatches to the guide RNA strand. We expected that this low specificity could be exploited to...
Topics
- Base Sequence
- CRISPR-Cas Systems
- Clustered Regularly Interspaced Short Palindromic Repeats
- Cyclin-Dependent Kinases
- Gene Knockout Techniques
- Genetic Engineering
- Mutagenesis
- Mutation
- Oryza
- Plant Proteins
- RNA, Guide, CRISPR-Cas Systems