Multigene knockout utilizing off-target mutations of the CRISPR/Cas9 system in rice.

Abstract excerpt

The clustered regularly interspaced short palindromic repeat (CRISPR)-associated endonuclease 9 (CRISPR/Cas9) system has been demonstrated to be a robust genome engineering tool in a variety of organisms including plants. However, it has been shown that the CRISPR/Cas9 system cleaves genomic DNA sequences containing mismatches to the guide RNA strand. We expected that this low specificity could be exploited to...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to RNA, Plant
• Share to Clustered Regularly Interspaced Short Palindromic Repeats