Article
Genome editing in rice and wheat using the CRISPR/Cas system.
Nature protocols - 1 Oct 2014
Shan Qiwei, Wang Yanpeng, Li Jun, Gao Caixia
Abstract excerpt
Targeted genome editing nucleases, such as zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs), are powerful tools for understanding gene function and for developing valuable new traits in plants. The clustered regularly interspersed short palindromic repeats (CRISPR)/Cas system has recently emerged as an alternative nuclease-based method for efficient and versatile genome...
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