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Article

Targeted mutagenesis in Arabidopsis thaliana using CRISPR-Cas12b/C2c1.

Journal of integrative plant biology - 1 Nov 2020

Wu Fan, Qiao Xinyu, Zhao Yafei, Zhang Ziyi, Gao Yifan, Shi Lingfeng, Du Haokun, Wang Lulu, Zhang Ya-Jie, Zhang Yu, Liu Langyu, Wang Quan, Kong Dejing

Abstract excerpt

Cas12b/C2c1 is a newly identified class 2 CRISPR endonuclease that was recently engineered for targeted genome editing in mammals and rice. To explore the potential applications of the CRISPR-Cas12b system in the dicot Arabidopsis thaliana, we selected BvCas12b and BhCas12b v4 for analysis. We successfully used both endonucleases to induce mutations, perform multiplex genome editing, and create large deletions at...

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PMID: 32396228
DOI: 10.1111/jipb.12944

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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