Article
Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice.
Nucleic acids research - 1 Nov 2013
Jiang Wenzhi, Zhou Huanbin, Bi Honghao, Fromm Michael, Yang Bing, Weeks Donald P
Abstract excerpt
The type II CRISPR/Cas system from Streptococcus pyogenes and its simplified derivative, the Cas9/single guide RNA (sgRNA) system, have emerged as potent new tools for targeted gene knockout in bacteria, yeast, fruit fly, zebrafish and human cells. Here, we describe adaptations of these systems leading to successful expression of the Cas9/sgRNA system in two dicot plant species, Arabidopsis and tobacco, and two...
Topics
- Arabidopsis
- CRISPR-Associated Proteins
- Endodeoxyribonucleases
- Gene Targeting
- Genes, Plant
- Genetic Engineering
- Genome
- Green Fluorescent Proteins
- Mutation
- Oryza
- Plant Leaves