Article
Rapid generation of genetic diversity by multiplex CRISPR/Cas9 genome editing in rice.
Science China. Life sciences - 1 May 2017
Shen Lan, Hua Yufeng, Fu Yaping, Li Jian, Liu Qing, Jiao Xiaozhen, Xin Gaowei, Wang Junjie, Wang Xingchun, Yan Changjie, Wang Kejian
Abstract excerpt
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated endonuclease 9 (CRISPR/Cas9) system has emerged as a promising technology for specific genome editing in many species. Here we constructed one vector targeting eight agronomic genes in rice using the CRISPR/Cas9 multiplex genome editing system. By subsequent genetic transformation and DNA sequencing, we found that the eight target...
Topics
- Base Sequence
- CRISPR-Cas Systems
- Gene Editing
- Genes, Plant
- Genetic Variation
- Genome, Plant
- Genotype
- Mutation
- Oryza
- Phenotype
- Plant Breeding