Article
A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants.
Molecular plant - 1 Aug 2015
Ma Xingliang, Zhang Qunyu, Zhu Qinlong, Liu Wei, Chen Yan, Qiu Rong, Wang Bin, Yang Zhongfang, Li Heying, Lin Yuru, Xie Yongyao, Shen Rongxin, Chen Shuifu, Wang Zhi, Chen Yuanling, Guo Jingxin, Chen Letian, Zhao Xiucai, Dong Zhicheng, Liu Yao-Guang
Abstract excerpt
CRISPR/Cas9 genome targeting systems have been applied to a variety of species. However, most CRISPR/Cas9 systems reported for plants can only modify one or a few target sites. Here, we report a robust CRISPR/Cas9 vector system, utilizing a plant codon optimized Cas9 gene, for convenient and high-efficiency multiplex genome editing in monocot and dicot plants. We designed PCR-based procedures to rapidly generate...
Topics
- Alleles
- Arabidopsis
- Base Sequence
- CRISPR-Cas Systems
- Gene Expression Regulation, Plant
- Genes, Plant
- Genetic Vectors
- Genome, Plant
- Inheritance Patterns