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Generating Mutant for a Stress-Responsive Gene in Rice Using CRISPR-Cas9 System.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2024

Prakash Chudamani Sharma, Sunkar Ramanjulu

Abstract excerpt

CRISPR/Cas9 system is one of the most often utilized engineering tools for genome editing in many organisms including crop plants and presents great value in both basic and applied research. This is a preferred method because of its relative simplicity, cost-effectiveness, and reliability. The Cas9 nuclease guided by a short single guide RNA (gRNA) can generate double-strand DNA breaks (DSB) at the specific sites...

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Identifiers

PMID: 38869803
DOI: 10.1007/978-1-0716-3973-3_20

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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