Article
Genome-wide discovery of drug-dependent human liver regulatory elements.
PLoS genetics - 1 Oct 2014
Smith Robin P, Eckalbar Walter L, Morrissey Kari M, Luizon Marcelo R, Hoffmann Thomas J, Sun Xuefeng, Jones Stacy L, Force Aldred Shelley, Ramamoorthy Anuradha, Desta Zeruesenay, Liu Yunlong, Skaar Todd C, Trinklein Nathan D, Giacomini Kathleen M, Ahituv Nadav
Abstract excerpt
Inter-individual variation in gene regulatory elements is hypothesized to play a causative role in adverse drug reactions and reduced drug activity. However, relatively little is known about the location and function of drug-dependent elements. To uncover drug-associated elements in a genome-wide manner, we performed RNA-seq and ChIP-seq using antibodies against the pregnane X receptor (PXR) and three active...
Topics
- Cells, Cultured
- Cytochrome P-450 CYP3A
- Gene Expression Regulation
- Genome, Human
- Hep G2 Cells
- Hepatocytes
- Histones
- Humans
- Liver
- Polymorphism, Single Nucleotide