Article
Computational and experimental investigation of constitutive behavior in AraC.
Proteins - 1 Dec 2014
Lowe Mary, Gullotti David, Damjanovic Ana, Cheng Ann, Dirla Stephanie, Schleif Robert
Abstract excerpt
Many mutations in the N-terminal arm of AraC result in constitutive behavior in which transcription of the araBAD genes occurs even in the absence of arabinose. To begin to understand the mechanism underlying this class of mutations, we used molecular dynamics with self-guided Langevin dynamics to simulate (1) wild-type (WT) AraC, (2) known constitutive mutants resulting from alterations in the regulatory arm,...
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