Article
Elucidating residue roles in engineered variants of AraC regulatory protein.
Protein science : a publication of the Protein Society - 1 Feb 2010
Tang Shuang-Yan, Cirino Patrick C
Abstract excerpt
The AraC regulatory protein was previously engineered to control gene expression specifically in response to D-arabinose and not the native effector L-arabinose (Tang et al., J Am Chem Soc 2008;130:5267-5271). Mutations were targeted in the ligand-binding pocket and on the AraC N-terminal arm, which plays an important role in maintaining repressing or activating conformations in the absence or presence of...
Topics
- AraC Transcription Factor
- Arabinose
- Binding Sites
- Gene Expression
- Mutation
- Promoter Regions, Genetic
- Protein Binding
- Protein Engineering