Article
Allosteric regulation within the highly interconnected structural scaffold of AraC/XylS homologs tolerates a wide range of amino acid changes.
Proteins - 1 Jan 2022
Picard Hunter R, Schwingen Kristen S, Green Lisa M, Shis David L, Egan Susan M, Bennett Matthew R, Swint-Kruse Liskin
Abstract excerpt
To create bacterial transcription "circuits" for biotechnology, one approach is to recombine natural transcription factors, promoters, and operators. Additional novel functions can be engineered from existing transcription factors such as the E. coli AraC transcriptional activator, for which binding to DNA is modulated by binding L-arabinose. Here, we engineered chimeric AraC/XylS transcription activators that...
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