DNA bending by AraC: a negative mutant.

Abstract excerpt

We sought a mutation in the DNA binding domain of the arabinose operon regulatory protein, AraC, of Escherichia coli that allows the protein to bind DNA normally but not activate transcription. The mutation was isolated by mutagenizing a plasmid overproducing a chimeric leucine zipper-AraC DNA bi...

Topics

• AraC Transcription Factor
• Bacterial Proteins
• DNA, Bacterial
• Escherichia coli
• Escherichia coli Proteins
• Leucine Zippers
• Mutation
• Nucleic Acid Conformation
• Protein Binding
• Repressor Proteins
• Transcription Factors