Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Mutations in the major outer membrane protein gene from Histophilus somni by an allelic exchange method.

Journal of microbiological methods - 1 Nov 2014

Ueno Yuichi, Hoshinoo Kaori, Tagawa Yuichi

Abstract excerpt

Functional roles of the major outer membrane protein (MOMP) gene from the bovine pathogen Histophilus somni have remained to be elucidated due to lack of mutagenesis methods easily applicable to this gene. In this study, the direct use of PCR-amplified mutated DNA flanking by an antibiotic selection marker for transformation of H. somni was applied to accomplish the site-directed mutagenesis via homologous...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacterial Outer Membrane Proteins
Share to Blood Bactericidal Activity

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Culture Media

Share to Genetics, Microbial

Share to Microbial Sensitivity Tests

Share to Molecular Biology

Share to Mutagenesis, Site-Directed

Share to Recombination, Genetic

Source

Identifiers

PMID: 25173953
DOI: 10.1016/j.mimet.2014.08.008

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Mutations in the major outer membrane protein gene from Histophilus somni by an allelic exchange method.PMID 25173953Read ↗

Serum resistance in Haemophilus parasuisSC096 strain requires outer membrane protein P2 expressionSemantic proximity Read ↗

Establishment of a Successive Markerless Mutation System in Haemophilus parasuis through Natural Transformation.Semantic proximity Read ↗

Efficient construction of Haemophilus parasuis mutants based on natural transformation.Semantic proximity Read ↗

A marker-free genetic manipulation method for G. parasuis strains developed by alternately culturing transformants at 37°C and 30°CSemantic proximity Read ↗

Evaluation of an isogenic major outer membrane protein-deficient mutant in the human model of Haemophilus ducreyi infection.Semantic proximity Read ↗

High-grade mutant OmpF induces decreased bacterial survival rate.Semantic proximity Read ↗

Site-directed mutations of the catalytic and conserved amino acids of the neuraminidase gene, nanH, of Clostridium perfringens ATCC 10543.Semantic proximity Read ↗

E. coli recA gene improves gene targeted homologous recombination in Mycoplasma hyorhinis.Semantic proximity Read ↗

A functional tonB gene is required for both utilization of heme and virulence expression by Haemophilus influenzae type b.Semantic proximity Read ↗

Outer membrane protein A of Escherichia coli contributes to invasion of brain microvascular endothelial cells.Semantic proximity Read ↗

Improved allelic exchange vectors and their use to analyze 987P fimbria gene expression.Semantic proximity Read ↗

The Role of luxS in Histophilus somni Virulence and Biofilm Formation.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.