Article
E. coli recA gene improves gene targeted homologous recombination in Mycoplasma hyorhinis.
Journal of microbiological methods - 1 May 2017
Ishag Hassan Z A, Xiong Qiyan, Liu Maojun, Feng Zhixin, Shao Guoqing
Abstract excerpt
Mycoplasma hyorhinis is an opportunistic pathogen of pigs. Recently, it has been shown to transform cell cultures, increasing the attention of the researchers. Studies on the pathogenesis require specific genetic tool that is not yet available for the pathogen. To address this limitation, we constructed two suicide plasmids pGEMT-tetM/LR and pGEMT-recA-tetM/LR having a tetracycline resistance marker flanked by...
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