Article

A marker-free genetic manipulation method for G. parasuis strains developed by alternately culturing transformants at 37°C and 30°C

2024-08-02

Abstract excerpt

<title>Abstract</title> <p>Background <italic>Glaesserella parasuis</italic> (<italic>G. parasuis</italic>) is a significant pathogen causing Glässer’s disease and substantial financial losses in swine production systems. However, studies on its gene function and pathogenesis are limited due to the lack of a broad marker-free knockout system. Currently, researchers mainly use antibiotic-resistant marked knockout...

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Literature Corpus work: bf143236-1925-5850-9057-8f65225caddf
DOI: 10.21203/rs.3.rs-4705811/v1

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