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Article

Establishment of a Successive Markerless Mutation System in Haemophilus parasuis through Natural Transformation.

PloS one - 1 Jan 2015

Zhang Luhua, Li Ying, Dai Ke, Wen Xintian, Wu Rui, Huang Xiaobo, Jin Jin, Xu Kui, Yan Qigui, Huang Yong, Ma Xiaoping, Wen Yiping, Cao Sanjie

Abstract excerpt

Haemophilus parasuis, belonging to the family Pasteurellaceae, is the causative agent of Glässer's disease leading to serious economic losses. In this study, a successive markerless mutation system for H. parasuis using two sequential steps of natural transformation was developed. By the first homologous recombination, the target genes were replaced by a cassette carrying kanamycin resistance gene and sacB (which...

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Identifiers

PMID: 25985077
DOI: 10.1371/journal.pone.0127393
PMCID: PMC4436007

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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