Article
Improved allelic exchange vectors and their use to analyze 987P fimbria gene expression.
Gene - 30 Jan 1998
Edwards R A, Keller L H, Schifferli D M
Abstract excerpt
A series of vectors has been developed to provide improved positive and negative selection for allelic exchange. Based on homologous regions of DNA ranging in size from less than 200 bp to over 1 kb, we have successfully used these new plasmids to introduce or remove markers in chromosomal or pla...
Topics
- Adhesins, Escherichia coli
- Alleles
- Antigens, Bacterial
- Antigens, Surface
- Bacterial Proteins
- Base Sequence
- Chloramphenicol O-Acetyltransferase
- DNA, Bacterial
- DNA-Binding Proteins
- Escherichia coli
- Fimbriae Proteins
- Fimbriae, Bacterial
- Gene Expression Regulation, Bacterial
- Genetic Vectors
- Iron
- Molecular Sequence Data
- Osmolar Concentration
- Recombinant Fusion Proteins