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Article

Efficient construction of Haemophilus parasuis mutants based on natural transformation.

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire - 1 Oct 2016

Li Junxing, Yuan Xiufang, Xu Lihua, Kang Lei, Jiang Jun, Wang Yicheng

Abstract excerpt

Studies on virulence factors and pathogenecity of Haemophilus parasuis have long been hindered by a lack of a consistent system for genetic manipulation. In this study, competence was induced by transferring H. parasuis from rich medium to starvation medium media-IV (M-IV) and iscR gene deficient mutants of H. parasuis were generated efficiently. Transformation frequency varied from 4.1 × 10-5 to 1.1 × 10-8 when...

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Identifiers

PMID: 27733782
PMCID: PMC5052879

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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