Site-directed mutations of the catalytic and conserved amino acids of the neuraminidase gene, nanH, of Clostridium perfringens ATCC 10543.

doi:10.1016/0141-0229(95)00245-6

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Article

Site-directed mutations of the catalytic and conserved amino acids of the neuraminidase gene, nanH, of Clostridium perfringens ATCC 10543.

Enzyme and microbial technology - 1 Sept 1996

Chien C H, Shann Y J, Sheu S Y

Abstract excerpt

The small nanH gene encoding the neuraminidase from Clostridium perfringens ATCC 10543 was cloned in JM109 using pUC19 as a vector. Sequence analysis revealed an ORF encoding 382 amino acids without a signal peptide sequence. Four regions of amino-acid sequence, 71-82, 140-151, 208-219, and 255-2...

Topics

Amino Acid Sequence
Base Sequence
Binding Sites
Blotting, Western
Chemical Phenomena
Chemistry
Cloning, Molecular
Clostridium perfringens
Conserved Sequence
DNA Primers
Genes, Bacterial
Kinetics

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Models, Molecular

Molecular Sequence Data

Mutagenesis, Site-Directed

Mutation

N-Acetylneuraminic Acid

Neuraminidase

Source

Open in PubMed

Identifiers

PMID: 8987487
DOI: 10.1016/0141-0229(95)00245-6

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8987487-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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