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Article

Labeled EF-Tus for rapid kinetic studies of pretranslocation complex formation.

ACS chemical biology - 17 Oct 2014

Liu Wei, Kavaliauskas Darius, Schrader Jared M, Poruri Kiran, Birkedal Victoria, Goldman Emanuel, Jakubowski Hieronim, Mandecki Wlodek, Uhlenbeck Olke C, Knudsen Charlotte R, Goldman Yale E, Cooperman Barry S

Abstract excerpt

The universally conserved translation elongation factor EF-Tu delivers aminoacyl(aa)-tRNA in the form of an aa-tRNA·EF-Tu·GTP ternary complex (TC) to the ribosome where it binds to the cognate mRNA codon within the ribosomal A-site, leading to formation of a pretranslocation (PRE) complex. Here we describe preparation of QSY9 and Cy5 derivatives of the variant E348C-EF-Tu that are functional in translation...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 25126896
DOI: 10.1021/cb500409y
PMCID: PMC4201349

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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