Article
Tuning the affinity of aminoacyl-tRNA to elongation factor Tu for optimal decoding.
Proceedings of the National Academy of Sciences of the United States of America - 29 Mar 2011
Schrader Jared M, Chapman Stephen J, Uhlenbeck Olke C
Abstract excerpt
To better understand why aminoacyl-tRNAs (aa-tRNAs) have evolved to bind bacterial elongation factor Tu (EF-Tu) with uniform affinities, mutant tRNAs with differing affinities for EF-Tu were assayed for decoding on Escherichia coli ribosomes. At saturating EF-Tu concentrations, weaker-binding aa-tRNAs decode their cognate codons similarly to wild-type tRNAs. However, tighter-binding aa-tRNAs show reduced rates of...
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