Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Two proofreading steps amplify the accuracy of genetic code translation.

Proceedings of the National Academy of Sciences of the United States of America - 29 Nov 2016

Ieong Ka-Weng, Uzun Ülkü, Selmer Maria, Ehrenberg Måns

Abstract excerpt

Aminoacyl-tRNAs (aa-tRNAs) are selected by the messenger RNA programmed ribosome in ternary complex with elongation factor Tu (EF-Tu) and GTP and then, again, in a proofreading step after GTP hydrolysis on EF-Tu. We use tRNA mutants with different affinities for EF-Tu to demonstrate that proofreading of aa-tRNAs occurs in two consecutive steps. First, aa-tRNAs in ternary complex with EF-Tu·GDP are selected in a...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acyl-tRNA Synthetases
Share to Genetic Code

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Guanosine Diphosphate

Share to Nucleic Acid Conformation

Share to Peptide Elongation Factor Tu

Share to Protein Biosynthesis

Share to Ribosomes

Share to RNA, Transfer

Source

Open in PubMed Open full text

Identifiers

PMID: 27837019
DOI: 10.1073/pnas.1610917113
PMCID: PMC5137768

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Two proofreading steps amplify the accuracy of genetic code translation.PMID 27837019Read ↗

Tuning the affinity of aminoacyl-tRNA to elongation factor Tu for optimal decoding.Semantic proximity Read ↗

Structural insights into translational fidelity.Semantic proximity Read ↗

tRNA residues evolved to promote translational accuracy.Semantic proximity Read ↗

An active role for tRNA in decoding beyond codon:anticodon pairing.Semantic proximity Read ↗

Multiple stages in codon-anticodon recognition: double-trigger mechanisms and geometric constraints.Semantic proximity Read ↗

Labeled EF-Tus for rapid kinetic studies of pretranslocation complex formation.Semantic proximity Read ↗

mRNA Translation Gone Awry: Translation Fidelity and Neurological Disease.Semantic proximity Read ↗

Cryo-EM shows stages of initial codon selection on the ribosome by aa-tRNA in ternary complex with GTP and the GTPase-deficient EF-TuH84A.Semantic proximity Read ↗

Role of tRNA amino acid-accepting end in aminoacylation and its quality control.Semantic proximity Read ↗

The Roots of Genetic Coding in Aminoacyl-tRNA Synthetase Duality.Semantic proximity Read ↗

GE2270A-resistant mutations in elongation factor Tu allow productive aminoacyl-tRNA binding to EF-Tu.GTP.GE2270A complexes.Semantic proximity Read ↗

Mutational unmasking of a tRNA-dependent pathway for preventing genetic code ambiguity.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.