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Article

Properties of Escherichia coli EF-Tu mutants designed for fluorescence resonance energy transfer from tRNA molecules.

Protein engineering, design & selection : PEDS - 1 Mar 2010

Perla-Kajan Joanna, Lin Xin, Cooperman Barry S, Goldman Emanuel, Jakubowski Hieronim, Knudsen Charlotte R, Mandecki Wlodek

Abstract excerpt

Here we describe the design, preparation and characterization of 10 EF-Tu mutants of potential utility for the study of Escherichia coli elongation factor Tu (EF-Tu) interaction with tRNA by a fluorescence resonance energy transfer assay. Each mutant contains a single cysteine residue at positions in EF-Tu that are proximal to tRNA sites within the aminoacyl-tRNA.EF-Tu.GTP ternary complex that have previously...

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Identifiers

PMID: 20083494
DOI: 10.1093/protein/gzp079
PMCID: PMC2816605

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