Article
Kinetically competent intermediates in the translocation step of protein synthesis.
Molecular cell - 23 Feb 2007
Pan Dongli, Kirillov Stanislav V, Cooperman Barry S
Abstract excerpt
Translocation requires large-scale movements of ribosome-bound tRNAs. Using tRNAs that are proflavin labeled and single-turnover rapid kinetics assays, we identify one or possibly two kinetically competent intermediates in translocation. EF-G.GTP binding to the pretranslocation (PRE) complex and GTP hydrolysis are rapidly followed by formation of the securely identified intermediate complex (INT), which is more...
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