Article
Discovery and characterization of artifactual mutations in deep coverage targeted capture sequencing data due to oxidative DNA damage during sample preparation.
Nucleic acids research - 1 Apr 2013
Costello Maura, Pugh Trevor J, Fennell Timothy J, Stewart Chip, Lichtenstein Lee, Meldrim James C, Fostel Jennifer L, Friedrich Dennis C, Perrin Danielle, Dionne Danielle, Kim Sharon, Gabriel Stacey B, Lander Eric S, Fisher Sheila, Getz Gad
Abstract excerpt
As researchers begin probing deep coverage sequencing data for increasingly rare mutations and subclonal events, the fidelity of next generation sequencing (NGS) laboratory methods will become increasingly critical. Although error rates for sequencing and polymerase chain reaction (PCR) are well documented, the effects that DNA extraction and other library preparation steps could have on downstream sequence...
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